Multiplexed electrospray deposition for protein microarraywith micromachined silicon device

• Published in: Applied physics letters Vol. 91; no. 1; pp. 014102 - 014102-3
• Main Author: Bhatnagar, Parijat
• Format: Journal Article
• Published: American Institute of Physics 05-07-2007
• ISSN: 0003-6951; 1077-3118
• DOI: 10.1063/1.2754642

Multiplexed electrospray deposition device capable of delivering picoliter volumes made by silicon micromachining technology has been developed as a deposition tool for making protein microarrays in a noncontact mode. Upon application of potential difference in the range of
7
-
9
kV
, biomolecules dissolved in suitable buffer with nonionic surfactant and loaded on the electrospray tips were dispensed on the substrate with microfabricated hydrogel features
(
1
-
10
μ
m
)
in cone-jet mode. Schiff base chemistry followed by reductive amination was utilized for covalent immobilization.