Cytotoxic cytochalasans from cultures of the fungus TBRC-BCC 79240

• Published in: RSC advances Vol. 13; no. 16; pp. 1564 - 1576
• Main Authors: Kornsakulkarn, Jittra, Auncharoen, Patchanee, Khonsanit, Artit, Boonyuen, Nattawut, Thongpanchang, Chawanee
• Format: Journal Article
• Published: 04-04-2023
• ISSN: 2046-2069
• DOI: 10.1039/d3ra00042g

Fourteen new cytochalasans, brunnesins A-N (
1-14
), along with eleven known compounds, were isolated from the culture extracts of the insect pathogenic fungus
Metarhizium brunneum
strain TBRC-BCC 79240. The compound structures were established by spectroscopy, X-ray diffraction analysis, and electronic circular dichroism. Compound
4
exhibited antiproliferative activity against all cell lines tested (mammalian), with 50% inhibition concentration (IC
50
) values ranging from 2.09 to 16.8 μg mL
−1
. Compounds
6
and
16
were shown to be bioactive only against non-cancerous Vero cells (IC
50
4.03 and 0.637 μg mL
−1
, respectively) whereas compounds
9
and
12
were bioactive only against NCI-H187 small-cell lung cancer cells (IC
50
18.59 and 18.54 μg mL
−1
, respectively). Compounds
7
,
13
, and
14
showed cytotoxicity against NCI-H187 and Vero cell lines with IC
50
values ranging from 3.98-44.81 μg mL
−1
.
Fourteen new cytochalasans, brunnesins A-N (
1-14
), along with eleven known compounds, were isolated from the culture extracts of the insect pathogenic fungus
Metarhizium brunneum
strain TBRC-BCC 79240.