Metal-based-oxide nanoparticles assisted the culture growth of as micronutrients: essential metabolic processes and genetic stability

• Published in: RSC advances Vol. 13; no. 17; pp. 11577 - 1159
• Main Authors: Ahmed, Mohamed F, Ibrahim, Mostafa A, Mansour, Ahmed S, Emam, Ahmed N, Abd El-Razik, Ashraf B, Tawfik, Eman
• Format: Journal Article
• Published: 13-04-2023
• ISSN: 2046-2069
• DOI: 10.1039/d2ra05941j

The present study evaluates the
in vitro
culture growth rate of
Populus alba
upon using nano metal-based-oxides such as hematite (Fe
2
O
3
NPs), zinc oxide (ZnO NPs), and manganese oxide (Mn
2
O
3
NPs) nanoparticles as analogues of three primary micronutrients such as iron (Fe), zinc (Zn), and manganese (Mn), which exist in soil as micronutrients. Herein, the
in vitro
culture growth rate was investigated using three different concentrations (
i.e.
, 20, 40, and 60 mg L
−1
) of as-prepared metal oxide nanoparticles compared to the control. In addition, the as-prepared nanoparticles have been prepared
via
the co-precipitation method. Furthermore, the physicochemical properties were investigated using transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and dynamic light scattering techniques. Overall, a significant difference in the biomass production-related parameters such as fresh weight, shoot length, and root length was observed compared to the control upon the treatment with micronutrient-based nano-metal-oxides (
i.e.
, Mn
2
O
3
> Fe
2
O
3
> ZnO NPs, respectively). In addition, a significant increase in the root number of
Populus alba
plants upon their treatment with ZnO NPs was observed compared to other prepared nano-metal-oxides and the control. Also, a remarkable increase in the chlorophyll index was monitored upon the treatment with Fe
2
O
3
NPs rather than the other commonly used Mn
2
O
3
and ZnO NPs, respectively. Moreover, RAPD-PCR bioassays were applied, and the actual six primers showed a genetic variation percentage of 34.17%, indicating that
Populus alba
is highly genetically stable even in highly contaminated soil. As a result, our findings suggest an idea that indicates the ability to enhance the
in vitro
culture growth rate of
Populus alba
plants using metal oxide nanoparticles as analogous to essential micronutrients.
In vitro
culture growth rate of
Populus alba
upon using nano metal-based-oxides such as hematite (Fe
2
O
3
NPs), zinc oxide (ZnO NPs), and manganese oxide (Mn
2
O
3
NPs) nanoparticles as micronutrients.