The (Himalayan columbine) mediated synthesis of nanoceria for diverse biomedical applications

• Published in: RSC advances Vol. 1; no. 33; pp. 19219 - 19231
• Main Authors: Jan, Hasnain, Khan, Muhammad Aslam, Usman, Hazrat, Shah, Muzamil, Ansir, Rotaba, Faisal, Shah, Ullah, Niamat, Rahman, Lubna
• Format: Journal Article
• Published: 20-05-2020
• ISSN: 2046-2069
• DOI: 10.1039/d0ra01971b

Herein, we report an eco-friendly, facile, one-pot, green synthesis of nanoceria for multiple biomedical applications. In the study, cerium oxide nanoparticles (CeO
2
-NPs) were synthesized using a simple aqueous extract of
Aquilegia pubiflora
as an effective reducing and capping agent. The biosynthesized nanoparticles were characterized
via
UV-vis spectroscopy, X-ray powder diffraction (XRD), high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Raman spectroscopy. The NPs were highly stable, exhibited high purity, and had a spherical morphology and mean size of 28 nm. FTIR and HPLC studies confirmed the successful capping of bioactive compounds on the nanoparticles. The well-characterized NPs were evaluated for a number of biomedical applications, and their antimicrobial (antifungal, antibacterial, and antileishmanial), protein kinase inhibition, anticancer, antioxidant, anti-diabetic and biocompatibility properties were studied. Our results showed that the
Aquilegia pubiflora
mediated CeO
2
-NPs were highly active against fungal strains, compared to the tested bacterial strains, with
Aspergillus niger
resulting in the largest zone of inhibition (15.1 ± 0.27 mm). The particles also exhibited dose dependent leishmanicidal activity with significant LC
50
values toward both the amastigote (114 μg mL
−1
) and promastigote (97 μg mL
−1
) forms of the parasite
Leishmania tropica
(KWH23). The NPs were found to be moderately active against the HepG2 cell line, showing 26.78% ± 1.16% inhibition at 200 μg mL
−1
. Last but not least, their highly biocompatible nature was observed with respect to freshly isolated human red blood cells (hRBCs), making the greenly synthesized CeO
2
-NPs a novel candidates for multidimensional medical applications.
Graphical illustration of eco-friendly, facile, one-pot, green synthesis of nanoceria for multiple biomedical applications.