MIRG2010 Study: Molecular Interactions in a Three Component System

• Published in: Journal of biomolecular techniques Vol. 21; no. 3 Suppl; p. S15
• Main Authors: Bergqvist, S., Edavettal, S., Eisenstein, E., Robinson, M., Yadav, S., Yamniuk, A., Yeung, A., Doyle, M.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Association of Biomolecular Resource Facilities 01-09-2010
• Subjects: Research Group Session Abstracts
• ISSN: 1524-0215; 1943-4731

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The MIRG 2010 Study was designed to benchmark the capabilities of different laboratories to determine whether two proteins that bind to a common third protein do so synergistically, competitively, or independently. This is a common question that arises during the discovery of protein therapeutic candidates, where it needs to be determined if the candidates bind to the same location on the target molecule or not.It is also a common question from proteomic pull-down and other types of experiments, where multiple proteins are identified as “interacting,” but it can not be determined from that data alone whether the proteins form a ternary complex or bind competitively.As Chair of the MIRG, I'll make a few introductory remarks about the project, and turn the podium over to the MIRG speakers who will give an update on the MIRG 2010 Study.