Phytochemical analysis, evaluation of the antioxidant and anti alzheimer activities of Algerian Seseli Trotuosum

• Published in: Chemistry & biodiversity p. e202400482
• Main Authors: Slimani, Abdelkader, Akkal, Wail, Benmekhbi, Ahmed Acheraf, Mosbah, Asma, Cakmak, Yavuz Selim, Lefahal, Mostefa, Bousetla, Ahlem, Mokrani, El Hassen, Bensouici, Chawki, Laoue, Hocine, Akkal, Salah, Nieto Martínez, Gema
• Format: Journal Article
• Language: English
• Published: Switzerland 17-09-2024
• Subjects: in silico study; anti-Alzheimer activity; Antioxidant activity; Seseli Trotuosum; Phytochemical Analysis
• ISSN: 1612-1880

HPLC analysis, phytochemical screening, thin layer chromatography, polyphenols and flavonoid contents were conducted to determine the bioactive contents of the Algerian Seseli tortuosum plant. Antioxidant activity was tested using DPPH and ABTS scavenging assays, reducing power, phenanthroline and silver nanoparticle (SNP) assays. BChE inhibitory assay was performed in vitro and in silico. Phytochemical analysis highlighted the richness of the extracts in terms of coumarins and terpenoids. The quantitative determination of total polyphenols and flavonoids showed that the highest amounts occurred in the dichloromethane (DCME) and methanolic (MeOH) extracts. The antioxidant activities indicated a moderate potential. Compared with galantamine, DCME had a significantly greater inhibitory effect on BChE (CI50 = 9.14±1.74 μg/ml and 34.75±1.99 μg/ml respectively). An in silico study of butyrylcholinesterase inhibition revealed a significant effect of quercetin (-30,13 KJ/mol). Conclusion: This study demonstrated the richness of the phytochemical components of seseli tortuosum, which are responsible for several biological properties, mainly their anti-Alzheimer potential.