Effects and mechanism of astragalus polysaccharide on wound healing of deep partial-thickness burns in rats

• Published in: Zhonghua shao shang yu chuang mian xiu fu za zhi Vol. 39; no. 3; p. 256
• Main Authors: Zheng, F, Cai, Y E, Li, L, Han, D W
• Format: Journal Article
• Language: Chinese
• Published: China 20-03-2023
• Subjects: Animals; Burns - drug therapy; Epidermal Growth Factor; Glycogen Synthase Kinase 3 beta; Interferon-gamma; Interleukin-2; Male; Polysaccharides - pharmacology; Proto-Oncogene Proteins c-akt; Rats; Rats, Sprague-Dawley; RNA, Messenger; Tumor Necrosis Factor-alpha; Water; Wound Healing
• ISSN: 2097-1109

To investigate the effects and mechanism of astragalus polysaccharide (APS) on wound healing of deep partial-thickness burns in rats.
The experimental study method was used. Fifty 7-week-old male Sprague-Dawley rats were divided into normal group, simple burn group, APS group, inhibitor group, and inhibitor+APS group according to the random number table, with 10 rats in each group. Except for normal group, rats in the other 4 groups were inflicted with a deep partial-thickness burn wound on the back. Rats in normal group and simple burn group were intraperitoneally injected with normal saline, and rats in the other three groups were injected with APS and/or integrin-linked kinase (ILK) inhibitor, respectively. After 7 days of injection, the wound healing rate of rats with burns in the four groups was calculated, and the serum content of interferon-γ, interleukin-2 (IL-2), and tumor necrosis factor α (TNF-α) in rats in 5 groups was determined by enzyme-linked immunosorbent assay (ELISA). The normal skin tissue