A Novel Multicellular Placental Barrier Model to Investigate the Effect of Maternal Aflatoxin B 1 Exposure on Fetal-Side Neural Stem Cells

• Published in: Toxins Vol. 15; no. 5
• Main Authors: Zhou, Zhiwei, Luo, Dongmei, Li, Mengxue, Lao, Guangjie, Zhou, Zhiqiang, Dinnyés, András, Xu, Wenming, Sun, Qun
• Format: Journal Article
• Language: English
• Published: Switzerland 27-04-2023
• Subjects: Aflatoxin B1 - metabolism; Aflatoxin B1 - toxicity; Animals; DNA Damage; Female; Humans; Liver - metabolism; Mycotoxins - metabolism; Placenta - metabolism; Pregnancy; multicellular in vitro model; neural stem cells; DNA damage; placental barrier
• ISSN: 2072-6651

Ingestion of food toxins such as aflatoxin B
(AFB
) during pregnancy may impair fetal neurodevelopment. However, animal model results may not be accurate due to the species' differences, and testing on humans is ethically impermissible. Here, we developed an in vitro human maternal-fetal multicellular model composed of a human hepatic compartment, a bilayer placental barrier, and a human fetal central nervous system compartment using neural stem cells (NSCs) to investigate the effect of AFB
on fetal-side NSCs. AFB
passed through the HepG2 hepatocellular carcinoma cells to mimic the maternal metabolic effects. Importantly, even at the limited concentration (0.0641 ± 0.0046 μM) of AFB
close to the national safety level standard of China (GB-2761-2011), the mixture of AFB
crossing the placental barrier induced NSC apoptosis. The level of reactive oxygen species in NSCs was significantly elevated and the cell membrane was damaged, causing the release of intracellular lactate dehydrogenase (
< 0.05). The comet experiment and γ-H2AX immunofluorescence assay showed that AFB
caused significant DNA damage to NSCs (
< 0.05). This study provided a new model for the toxicological evaluation of the effect of food mycotoxin exposure during pregnancy on fetal neurodevelopment.