Molecular composition of the fractions of immunoglobulin preparations studied by gel filtration

Molecular composition of the fractions of immunoglobulin preparations studied by gel filtration

The method of gel filtration in columns permitted the separation of aggregated fractions into polymers whose content did not exceed 10% and dimers, their content ranging from 3.6% to 22.11%. The preparations were also found to contain fractions of monomers and fragments, Fab-fragments being detected...

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Bibliographic Details
Published in:Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 12; p. 74
Main Authors: Kargina, T M, Runova, V F, Gavrilenkova, V Iu
Format: Journal Article
Language:Russian
Published: Russia (Federation) 01-12-1987
Subjects:
Chromatography, Gel - methods
Drug Storage
Humans
Immunoglobulin Fragments - analysis
Immunoglobulin G - analysis
Immunoglobulin G - standards
Spectrophotometry, Ultraviolet
Time Factors
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Summary:The method of gel filtration in columns permitted the separation of aggregated fractions into polymers whose content did not exceed 10% and dimers, their content ranging from 3.6% to 22.11%. The preparations were also found to contain fractions of monomers and fragments, Fab-fragments being detected in 10 out of 20 batches under study (4.04-27.36%). The shelf life of all preparations did not exceed 8-12 months. The use of spectrophotometric techniques ensured obtaining the most objective results in the calculation of the percentage of fractions contained in immunoglobulin preparations. The evaluation of the molecular composition of immunoglobulin preparations by the method of gel filtration is conducive to the improvement of their quality.
ISSN:0372-9311