The effect of hyperglycemia on indices of biochemical blood analysis in vitro.

• Published in: Klinicheskaia laboratornaia diagnostika Vol. 63; no. 4; p. 205
• Main Authors: Gilmiyarova, F N, Gusyakova, O A, Baldina, O A, Ereschenko, A A, Mezhenkova, I A, Archibasova, O V, Khaliulin, A V, Sosnovskaya, L V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2018
• Subjects: Alanine Transaminase - blood; Aspartate Aminotransferases - blood; Bilirubin - blood; Blood Chemical Analysis; Blood Glucose - analysis; Creatinine - blood; Diabetes Mellitus; Humans; Hyperglycemia - blood; L-Lactate Dehydrogenase - blood; Urea - blood; personified medicine; hyperglycemia; blood group AB0; diabetes mellitus; interference; biochemical analysis of blood; post-analytical stage of laboratory analysis
• ISSN: 0869-2084

The purpose of study was to detect possible deviations between the results of biochemical analysis of blood and true values in conditions of hyperglycemia in vitro and dependences of the given alterations from group belonging of analyzed blood. The biochemical analysis was applied to blood samples of patients with various blood groups according system AB0 in conditions of normo- and hyperglycemia in vitro. The criterion for choice was established the content of main biochemical indices of blood (total protein, total bilirubin, glucose, total cholesterol, urea, creatinine, activity of hylaninamitotransferase (ALT), aspartataminotransferase (AST), lactatedehydrogenase (LDG)) within the reference limits. The hyperglycemia was developed in vitro using 5% glucose solution and corresponded to 20,49±0,05 and 46,37±0,63 mmol/l. The content of total protein and total cholesterol turned out to be the most stable indices. The effect of increased level of glucose to following analytes was established: total bilirubin (+10,5%), ALT activity (±13,4%), AST activity (+11,1%) and creatinine (+51,4%). The alterations of indices up to level of ±10% and higher are clinically significant and have to be taking into account by physicians of clinical laboratory diagnostic and clinical physicians during interpretations of the results of biochemical analysis of blood for evaluation of true picture of condition of patient. The AB0-group specific characteristics of biochemical analysis of blood are established in conditions of hyperglycemia. The hyperglycemia is an interfering factor during implementation of laboratory analysis that is especially actual at interpretations of results of biochemical analysis of blood in patients of reanimation department and individuals suffering of persistent and severe diabetes mellitus with its complication with nephropathy. Besides, the established group-specific characteristics of hyperglycemia effecting the results of biochemical analysis of blood can be accounted in development of standards and guidelines of personified medicine.