Detection of Legionella pneumophila antibodies in the blood serum of pneumonia patients by an immunoenzyme method

The conditions of making the enzyme immunoassay (EIA) for the detection of antibodies to L. pneumophila have been optimized. The use of L. pneumophila purified serotypic antigen at a concentration of 0.25 micrograms/ml for the sensitization of polystyrene plates has been shown to increase the sensit...

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Bibliographic Details
Published in:	Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 11; p. 79
Main Authors:	Tartakovskiĭ, I S, Radchenko, O V, Rusakova, E V, Belen'kiĭ, S N, Prozorovskiĭ, S V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-1988
Subjects:	Antibodies, Bacterial - analysis Antibodies, Bacterial - isolation & purification Antibody Specificity Evaluation Studies as Topic Fluorescent Antibody Technique Humans Immunoenzyme Techniques - instrumentation Legionella - immunology Legionnaires' Disease - diagnosis Pneumonia - diagnosis
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Summary:	The conditions of making the enzyme immunoassay (EIA) for the detection of antibodies to L. pneumophila have been optimized. The use of L. pneumophila purified serotypic antigen at a concentration of 0.25 micrograms/ml for the sensitization of polystyrene plates has been shown to increase the sensitivity and specificity of the assay. 220 patients with severe pneumonia have been examined. As revealed in this investigation, antibodies to L. pneumophila can be detected in 12.2% of cases. A high degree of correlation (94.4%) between the results of EIA and the indirect immunofluorescence test has been shown.
ISSN:	0372-9311