In Vitro Anti-mycobacterial Activity of Three Medicinal Plants of Lamiaceae Family

• Published in: Recent patents on anti-infective drug discovery Vol. 13; no. 3; p. 240
• Main Authors: Kazemian, Hossein, Heidari, Hamid, Yamchi, Jalil Kardan, Zandi, Hengameh, Taji, Asieh, Yazdani, Fateme, Hamzehloo, Gholamreza, Ghanavati, Roya, Rahdar, Hossein A, Feizabadi, Mohammad M
• Format: Journal Article
• Language: English
• Published: Netherlands 2018
• Subjects: Antitubercular Agents - pharmacology; Antitubercular Agents - therapeutic use; Humans; Iran; Lamiaceae - chemistry; Microbial Sensitivity Tests; Mycobacterium Infections, Nontuberculous - drug therapy; Mycobacterium Infections, Nontuberculous - microbiology; Mycobacterium tuberculosis - drug effects; Nontuberculous Mycobacteria - drug effects; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plants, Medicinal - chemistry; Tuberculosis, Multidrug-Resistant - drug therapy; Tuberculosis, Multidrug-Resistant - microbiology; Iran; Satureja rechingeri; Satureja khuzestanica; essential oil; Anti-mycobacterial; Zataria multiflora; novel drugs
• ISSN: 2212-4071

Mycobacterium tuberculosis as an intracellular pathogen causes Tuberculosis (TB). Due to the long time required for treatment, hepatotoxicity of drugs and also emergence of Multidrug-Resistant (MDR) and Extremely Drug Resistant (XDR) strains, TB is currently a major public health problem. Some medicinal plants possess remarkable activity against Mycobacterium. Among them, Lamiaceae family are of pharmaceutical interest because of their potential antimicrobial properties. The aim of the study was to evaluate the in vitro activities of Satureja rechingeri, Satureja khuzestanica and Zataria multiflora against MDR M. tuberculosis and two Non-Tuberculous Mycobacteria (NTM).
The essential oils were prepared by the standard method. The confirmed strains were obtained from the microbial collection of Tehran University of Medical Sciences. Minimum Inhibitory Concentrations (MICs) of essential oils of plants against mycobacterial strains were determined using standard broth microdilution method.
MDR M. tuberculosis was completely inhibited by Z. multiflora at 78µg/ml concentration. S. rechingeri and S. khuzestanica also showed same anti-mycobacterial activity against MDR M. tuberculosis with MICs of 156 µg/ml. The MICs of the essential oils against M. tuberculosis H37Rv, M. kansasii and M. fortuitum were in the range from 39 to 156 µg/ml.
The studied medicinal plants showed notable effects against mycobacterial strains. Our results indicated that utilization of Lamiaceae family can be helpful for treatment of mycobacterial infections.