Isolation of bla NDM -producing Enterobacteriaceae in a public hospital in Salvador, Bahia, Brazil

• Published in: The Brazilian journal of infectious diseases
• Main Authors: Barberino, Maria Goreth, Cruvinel, Silvia de Araujo, Faria, Célio, Salvino, Marco Aurélio, Silva, Marcio de Oliveira
• Format: Journal Article
• Language: English
• Published: Brazil 13-11-2017
• Subjects: Carbapenemase; Antimicrobial resistance; New Delhi Metallo-b-lactamase
• ISSN: 1678-4391

Carbapenemases have great importance in the global epidemiological scenario since infections with carbapenemase-producing bacteria are associated with high mortality, especially in hospitalized patients in intensive care units. This study describes two microorganisms producers of the New Delhi Metallo-b-lactamase, Klebsiella pneumoniae and Citrobacter freundii, from two patients admitted to a public hospital in Salvador, Bahia. These are the first clinical cases of New Delhi Metallo-b-lactamase described in microorganisms in the north and northeast Brazil. The isolates were characterized by antimicrobial susceptibility test, with resistance to all β-lactams including carbapenems, negative Modified Hodge Test and the synergy test with Ethylenediaminetetraacetic acid, Phenylboronic Acid and Cloxacillin was positive only with Ethylenediaminetetraacetic acid (difference of >5mm in the inhibition zone between the disk without and with the inhibitor). Analysis by multiplex PCR for bla
, bla
, bla
, bla
and bla
enzymes confirmed the presence of bla
gene. This report of two different New Delhi Metallo-b-lactamase-producing microorganisms in a different region of Brazil confirms the risk of spreading resistance genes between different species and emphasizes the need for prevention and control of infections caused by these pathogens, which have limited treatment options and have been linked to high mortality rates.