Intralobular Distribution of Class I Alcohol Dehydrogenase and Aldehyde Dehydrogenase 2 Activities in the Hamster Liver

The hepatic lobular localization of class I alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) 2 activities was examined histochemically using livers of hamsters with high ethanol preferences. The activity of class I ADH detected by the nitro blue tetrazolium method using 5 mM ethanol as...

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Bibliographic Details
Published in:	Alcoholism, clinical and experimental research Vol. 23 Suppl s4; p. 52S
Main Authors:	Watabiki, Toshimitsu, Tokiyasu, Takuma, Yoshida, Manabu, Okii, Yutaka, Yoshimura, Sumitaka, Akane, Atsushi
Format:	Journal Article
Language:	English
Published:	England 01-04-1999
Subjects:	Class I Alcohol Dehydrogenase Alcohol Preference Aldehyde Dehydrogenase 2 Hamster Histochemistry
Online Access:	Get full text
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Summary:	The hepatic lobular localization of class I alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) 2 activities was examined histochemically using livers of hamsters with high ethanol preferences. The activity of class I ADH detected by the nitro blue tetrazolium method using 5 mM ethanol as a substrate was extremely high and was almost homogeneously distributed throughout the lobule. The ALDH 2 activity (substrate, 8 μM acetaldehyde) was localized to the centrilobular zone, whereas low K ALDH (ALDH 1 + ALDH 2) activity (substrate, 50 μM acetaldehyde) showed a gradient distribution in the lobule with high centrilobular to moderate periportal activity, suggesting that the ALDH 1 activity was distributed throughout the lobule.
ISSN:	1530-0277