Isolation of tick-borne borreliosis agent from blood of patients

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 1; p. 63
• Main Authors: Nefedova, V V, Teterin, V Iu, Korenberg, E I, Gorelova, N B, Vorob'eva, N N, Frizen, V I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-01-2009
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Borrelia - classification; Borrelia - genetics; Borrelia - isolation & purification; Borrelia Infections - diagnosis; Culture Media; DNA, Bacterial - analysis; DNA, Bacterial - blood; DNA, Bacterial - urine; Female; Humans; Male; Middle Aged; Molecular Sequence Data; Polymerase Chain Reaction; Russia; Tick-Borne Diseases - diagnosis; Tick-Borne Diseases - microbiology; Russia
• ISSN: 0372-9311

During spring-autumn period of 2006 Borrelia were isolated for the first time in Russia from blood of 79 patients treated in Perm City Clinical Hospital for Infectious Diseases No. 1 with diagnosis "tickborne borreliosis, manifestive form with migrating erythema, localized stage". Ten primary isolates (12.7% of total seeded samples) were obtained by seeding plasma samples on the BSK medium. Their subsequent identification by polymerase chain reaction-restriction fragments length polymorphism revealed presence of Borrelia garinii NT29 in all patients. Length of sequenced fragment of rrfA-rrlB region was 253 b.p. Seven isolates had 100% and 3 - 99.6% similarity with typical strain NT29 (L30130). Nucleotide sequences of 4 obtained isolates were deposited in GenBank database (No. AM932199 - AM932202). It was proposed that B. garinii NT29 more frequently than other Borrelia species can be an etiologic agent of tick-borne borreliosis not only in Perm region but also in whole Russia.