Preliminary data concerning cross-reacting anticardiolipin antibodies as potential inhibiting endotoxin activity factors

• Published in: Polskie archiwum medycyny wewne̦trznej Vol. 115; no. 5; p. 452
• Main Authors: Zabek, Jakub, Noworyta, Jacek, Brasse-Rumin, Maria, Pyka, Joanna, Krzewska, Iwona, Rell-Bakalarska, Maria
• Format: Journal Article
• Language: Polish
• Published: Poland 01-05-2006
• Subjects: Antibodies, Anticardiolipin - analysis; Antibodies, Anticardiolipin - immunology; Antibodies, Bacterial - analysis; Antibodies, Bacterial - immunology; Antigens, Bacterial - analysis; Antigens, Bacterial - immunology; Arthritis - immunology; Arthritis, Rheumatoid - immunology; Arthritis, Rheumatoid - microbiology; Cross Reactions; Humans; Limulus Test - methods; Lipopolysaccharides - antagonists & inhibitors; Lipopolysaccharides - immunology; Osteoarthritis - immunology; Osteoarthritis - microbiology; Spondylarthritis - immunology; Spondylarthritis - microbiology; Synovial Fluid - immunology
• ISSN: 1897-9483

In analyzed synovial fluids, suspected for endotoxin presence, the presence of endotoxin done was proved by LAL-test in 21 synovial fluids /42%/ and in 12 of 20 /60%/ the presence of endotoxin inhibiting substances /IS/ was proved. Because many parameters /like presence CIC, anti-LPS, KDO/ indicates that in the group of LAL-negative /IS/ - positive synovial fluids the presence of endotoxin is very possible, IS have been removed /by heating to 100 degrees C/ and after that the endotoxin activity was shown in about 27% of synovial fluids. Additional analysis of the Cohn fraction /prepared by ammonium sulfate precipitation/ confirmed the presence in the gamma and alpha and beta fractions a weak IS activity /in a dose dependent manner. No IS activity, have been shown yet in the fraction of affinity purified aCl antibodies.