Klebsiella pneumoniae secreted protein-containing antigens in the system of adaptive immunity

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 1; p. 51
• Main Authors: Voiushin, K E, Trishin, A V, Donenko, F V, Iastrebova, N E, Vaneeva, N P, Kiselevskiĭ, M V, Egorova, N B, Kyrbatova, E A, Semenova, I B, Semenov, B F
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-01-2006
• Subjects: Animals; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Antibody Specificity; Antigens, Bacterial - chemistry; Antigens, Bacterial - immunology; Antigens, Bacterial - metabolism; Humans; Immunization; Klebsiella Infections - blood; Klebsiella pneumoniae - immunology; Klebsiella pneumoniae - metabolism; Mice; Molecular Weight; Spondylitis, Ankylosing - blood
• ISSN: 0372-9311

The study was aimed at the evaluation of the antigenic properties of K. pneumoniae secreted protein-containing antigens with a molecular weightt of 21 and 34-35 kD, obtained from supernatant culture fluid. As confirmed by the method of flow cytofluorimetry, the protein-containing fractions belonged to the secreted components of the microbial cell. The fraction with a molecular weight of 34-35 kD possessed high antigenic activity and contributed to the formation of specific antibodies after the immunization of mice. At the same time none of the protein fractions lead to an increase in the level of autoantibodies in mouse blood sera to organ-unspecific and organ-specific antigens. As revealed by the method of solid-phase, in 6 (27.3%) from 22 patients of patients with rhizomelic spondylitis had an increased level of IgG to K. pneumoniae cell-wall antigens with a molecular weight of 34-35 kD. An increase in the level of IgG to the secreted protein-containing fraction with a molecular weight of 34-35 kD was detected only in one patient (4.5%) (p<0.05).