Modified proteinases in peptide synthesis in organic media

We showed that modified proteases could catalyze synthesis of a wide variety of peptides of various lengths and structures both in solution and on solid phase in organic solvents. The following modified proteases were studied as catalysts for enzymatic peptide synthesis in polar organic solvents (ac...

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Bibliographic Details
Published in:	Bioorganicheskaia khimiia Vol. 29; no. 5; p. 544
Main Authors:	Filippova, I Iu, Lysogorskaia, E N
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-09-2003
Subjects:	Endopeptidases - chemistry Organic Chemicals - chemistry Peptides - chemical synthesis
Online Access:	Get more information
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Summary:	We showed that modified proteases could catalyze synthesis of a wide variety of peptides of various lengths and structures both in solution and on solid phase in organic solvents. The following modified proteases were studied as catalysts for enzymatic peptide synthesis in polar organic solvents (acetonitrile, dimethylformamide, and ethanol): pepsin sorbed on celite, a noncovalent complex of subtilisin with sodium dodecylsulfate, and subtilisin or thermolysin covalently immobilized on a cryogel of polyvinyl alcohol. The use of the noncovalent complex of subtilisin with sodium dodecylsulfate and immobilized subtilisin is especially promising for the segment condensation of peptide fragments containing residues of trifunctional amino acids with unprotected ionogenic groups in side chains, such as Lys, Arg, His, Glu, and Asp.
ISSN:	0132-3423