Mechanism of propofol-induced apoptosis in neonatal rat oligodendrocytes

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 43; no. 10; pp. 1771 - 1775
• Main Authors: Ji, X, Zhu, Y, Zhang, D, Cai, Y, Zhou, G
• Format: Journal Article
• Language: Chinese
• Published: 20-10-2023
• ISSN: 1673-4254
• DOI: 10.12122/j.issn.1673-4254.2023.10.15

OBJECTIVETo explore the molecular mechanism of propofol-induced apoptosis in oligodendrocytes.METHODSFortyfive neonatal (7 days old) SD rats were randomized into 3 groups (n=15) for a single intraperitoneal injection of saline (control), long chain fat emulsion, or propofol (50 mg/kg). Eight hours after the injection, the rats were examined for mRNA and protein expressions of caspase-3, caspase-8, and caspase-9 in the brain tissues using qPCR and Western blotting, and the expression levels of nerve growth factor (NGF) mRNA and P-PI3K/P-PAkt were also detected.RESULTSCompared with those in the control group, the neonatal rats with propofol injection showed significantly up-regulated mRNA and protein expressions of caspase-3, caspase-8, and caspase-9 in the brain tissue (P<0.05) with significantly down-regulated expressions of NGF mRNA and P-PI3K/P-PAkt (P<0.05).CONCLUSIONPropofol induces apoptosis in oligodendrocytes by activating the caspase family protein members involved in triggering cell apoptosis and inh