IDENTIFICATION OF Glu-B1 ALLELES IN BREAD WHEAT CULTIVARS USING PCR
High dough strength is used as a predictor of good quality bread wheat and it has been attributed largely to the type of allele present at the Glu-D1 locus, where the Glu-D1d allele is most favorable. Recently, it has been observed that cultivars with over expressed subunit Bx7 super(0E) at the Glu-...
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| Published in: | Genetika (Beograd) Vol. 39; no. 1; pp. 23 - 28 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-01-2007
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | High dough strength is used as a predictor of good quality bread wheat and it has been attributed largely to the type of allele present at the Glu-D1 locus, where the Glu-D1d allele is most favorable. Recently, it has been observed that cultivars with over expressed subunit Bx7 super(0E) at the Glu-B1 have enhanced dough strength. In order to implement recently developed PCR assay for Glu-B1 x-type allele discrimination in marker-assisted selection, a set of wheat cultivars from 11 countries was analyzed. The PCR results matched the known Glu-B1 HMW GS genotypes of the cultivars possessing subunits Bx7 or Bx17. It was shown that this molecular marker also differentiated some other Glu-B1 alleles: Bx6, Bx13, Bx20 and Bx22. These results create the opportunity for an improved method of polymorphism scoring at the Glu-B1 in bread wheat germplasm. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2 |
| ISSN: | 0534-0012 |
| DOI: | 10.2298/GENSR07010230 |