MEMBRANE AND SYNAPTIC EFFECTS OF ANH-S-100 ARE PREVENTED BY THE SAME ANTIBODIES IN LOW CONCENTRATIONS

• Published in: Frontiers in bioscience Vol. 8; pp. 79 - 84
• Main Authors: Epstein, OI, Beregovoy, NA, Sorokina, N S, Starostina, M V, Shtark, M B, Gainutdinov, K L, Gainutdinova, T K, Muhamedshina, DI
• Format: Journal Article
• Language: English
• Published: 01-01-2003
• ISSN: 1093-9946

Effects of antiserum to S-100 protein (AS-100) in high (HC, corresponding to antiserum dilution 1:5 - 1:50) and low (LC, 1:10 super(12)) concentrations were studied in identified snail neurons and rat hippoeampal slices. HC-AS-1QQ changed the frequency of action potential generation in spontaneously active neurons and blocked formation of long-term potentiation in mossy fiber synapses. LC-AS-100 atone did not affect any characteristic measured in our experiments, but 20 min pre-incubation of snail ganglia or hippocampal slices with LC-AS-100 abolished the effects of the same antibodies in HC. Simultaneous addition of both LC and HC did not prevent the development of HC-AS-100 effects. It seems possible that LC-AS-100 is capable of interaction with neuronal cells modifying their reaction to HC-AS-100.