The antigen-specific memory CD8 super(+) T-cell response induced by BCG in cattle resides in the CD8 super(+) gamma / delta TCR super(-)CD45RO super(+) T-cell population

• Published in: Vaccine Vol. 27; no. 2; pp. 270 - 279
• Main Authors: Hogg, Alison E, Worth, Andrew, Beverley, Peter, Howard, Christopher J, Villarreal-Ramos, Bernardo
• Format: Journal Article
• Language: English
• Published: 01-01-2009
• Subjects: Mycobacterium bovis
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2008.10.053

Tuberculosis (TB) remains a worldwide leading cause of death among infectious diseases. Development of safer and more efficacious vaccines requires a basic understanding of the protective mechanisms induced by BCG. Here we show that vaccination of cattle with BCG induces CD8 super(+) gamma / delta TCR super(- )CD45RO super(+) T-cells that can produce IFN- gamma , up-regulate transcription and expression of perforin, lyse BCG-infected monocyte-derived macrophages (MoM[phi]) and contribute to a reduction in the number of intracellular mycobacteria. We also observed BCG-induced CD8 super(+) responses in vivo. After infection of cattle with Mycobacterium bovis, CD8 super(+) gamma / delta TCR super(- )CD45RO super(+) cells responded more strongly to M. bovis-infected MoM[phi] than to BCG-infected MoM[phi]. These results indicate that the antigen- specific CD8 super(+) memory response resides in the CD8 super(+) gamma / delta TCR super(- )CD45RO super(+) cell population.