PRIMER NOTE: Identification and characterization of nuclear microsatellites in Mediterranean cedars (Cedrus sp.)

• Published in: Molecular ecology notes Vol. 6; no. 3; pp. 840 - 842
• Main Authors: Chaib, J, Danan, S, Jouaud, B, Hagen, L S, Lefevre, F, Fady, B
• Format: Journal Article
• Language: English
• Published: 01-09-2006
• Subjects: Cedrus; Cedrus atlantica
• ISSN: 1471-8278; 1471-8286
• DOI: 10.1111/j.1471-8286.2006.01364.x

We developed primers for the amplification of six polymorphic nuclear microsatellites in Mediterranean Cedrus taxa. Microsatellites originated from two Cedrus atlantica genomic libraries enriched for TC (four markers) and TG (two markers) motifs. No distortion from Mendelian segregation was observed. There were up to eight alleles per locus and mean expected heterozygosity was 0.75. Four microsatellites also amplified in the Himalayan cedar. These markers should be helpful for species identification, diversity studies, parentage analysis and genome mapping and to monitor biodiversity in endangered Mediterranean Cedrus forests.