Novel yeast isolated from broilers' feedstuff, gut and faeces as aflatoxin B sub(1) adsorbents

• Published in: Journal of applied microbiology Vol. 121; no. 6; pp. 1766 - 1776
• Main Authors: Magnoli, A P, Rodriguez, M C, Poloni, V L, Rojo, M C, Combina, M, Chiacchiera, S M, Dalcero, A M, Cavaglieri, L R
• Format: Journal Article
• Language: English
• Published: 01-12-2016
• Subjects: Candida tropicalis; Clavispora; Pichia
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.13297

Aims To isolate and characterize native yeast strains from broilers' environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B sub(1) (AFB sub(1)). Methods and Results A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB sub(1) binding percentages varied among yeast strains and with AFB sub(1) concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 10 super(7) cells per ml and 100 ng ml super(-1) of AFB sub(1) respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB sub(1)-yeast complex demonstrated the irreversibility of the binding process. Conclusion Yeast strains tested in this study constitute potential AFB sub(1) adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study This study makes a contribution to using native yeasts from broilers' environment for controlling chronic aflatoxicosis in avian production.