Plant regeneration of creeping bluestem (Schizachyrium scoparium (Michx.) Nash var. stoloniferum (Nash) J. Wipff) via somatic embryogenesis

• Published in: In vitro cellular & developmental biology. Plant Vol. 37; no. 5; pp. 550 - 554
• Main Authors: Chakravarty, T, Norcini, J G, Aldrich, J H, Kalmbacher, R S
• Format: Journal Article
• Language: English
• Published: 01-10-2001
• Subjects: 2,4-dichlorophenoxyacetic acid; 6-benzylaminopurine; gibberellic acid; Schizachyrium scoparium; zeatin
• ISSN: 1054-5476
• DOI: 10.1079/IVP2001183

Creeping bluestem (Schizachyrium scoparium (Michx.) Nash var. stoloniferum (Nash) J. Wipff) embryogenic callus growing on solid medium was used to establish a cell suspension culture in Murashige and Skoog (MS) basal medium supplemented with 1.5 mg l super(-1) (6.8 mu M) 2,4-dichlorophenoxyacetic acid (2,4-D), 0.2 mg l super(-1) (0.88 mu M) 6-benzylaminopurine (BA), 0.5 mg l super(-1) (1.4 mu M) zeatin, 0.2 mg l super(-1) (0.58 mu M) gibberellic acid (GA sub(3)), and 10% (v/v) of coconut water (CW). Pro-embryos from suspension culture matured on semi-solid MS medium in about 18 wk, and were then cultured on semi-solid MS medium without growth regulators for 2-3 wk. Shoots were regenerated on MS basal medium supplemented with 3.0 mg l super(-1) (13.6 mu M) 2,4-D, 1.0 mg l super(-1) (4.4 mu M) BA, 1.0 mg l super(-1) (2.9 mu M) GA sub(3), 0.5 mg l super(-1) (2.7 mu M) 1-naphthaleneacetic acid (NAA), 500 mg l super(-1) casein hydrolysate, and 10% (v/v) CW. Rooted plantlets were successfully acclimatized to greenhouse and outdoor conditions. Using this protocol, it would be possible to produce at least 1300 fully acclimatized plantlets annually.