Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer: e0142006

• Published in: PloS one Vol. 10; no. 11
• Main Authors: Srinivas, Chatla, Ramaiah, M Janaki, Lavanya, A, Yerramsetty, Suresh, Kishor, P BKavi, Basha, Shaik Anver, Kamal, Ahmed, Bhadra, Utpal, Bhadra, Manika-Pal
• Format: Journal Article
• Language: English
• Published: 01-11-2015
• ISSN: 1932-6203
• DOI: 10.1371/journal.pone.0142006

Tumor microenvironment play role in angiogenesis and carcinogenesis. Etoposide, a known topoisomerase II inhibitor induces DNA damage resulting in cell cycle arrest. We developed a novel Etoposide analogue, Quinazolino-4[Beta]-amidopodophyllotoxin (C-10) that show better efficacy in regulating cell proliferation and angiogenesis. We evaluated its role on expression of microRNAs-15, 16, 17 and 221 and its targets Bcl-2, STAT3 and VEGF that dictate cell proliferation and angiogenesis. Docking studies clearly demonstrated the binding of Etoposide and C-10 to STAT3. We conclude that combination of Etoposide or C-10 with miR-15, 16, 17 and 221 as a new approach to induce apoptosis and control angiogenesis in breast cancer.