Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G sub(2) accumulation by a mechanism which differs from DNA damage checkpoint control
Vpr is a 96-amino-acid protein encoded by human immunodeficiency virus type 1 (HIV-1) that prevents proliferation of infected cells. We have established a system for infection of 100% of a T-cell population with HIV and use this system to show that within the context of HIV-1 infection, Vpr is prima...
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| Published in: | Journal of virology Vol. 70; no. 4; pp. 2324 - 2331 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-01-1996
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Vpr is a 96-amino-acid protein encoded by human immunodeficiency virus type 1 (HIV-1) that prevents proliferation of infected cells. We have established a system for infection of 100% of a T-cell population with HIV and use this system to show that within the context of HIV-1 infection, Vpr is primarily cytostatic rather than cytotoxic. Vpr acts upstream of dephosphorylation of the mitotic cyclin-dependent kinase, and causes infected cells to accumulate in the G sub(2) stage of the cell cycle. However, some HIV-1 infected cells increase in ploidy and size, accumulating DNA to an 8N level. Furthermore, the mechanism of the Vpr mitotic block is qualitatively different from that of G sub(2) DNA damage checkpoint control. |
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| Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
| ISSN: | 0022-538X |