Pp59 super(fyn) and pp62 super(c-yes) are enriched in SH-SY5Y neuroblastoma growth cones but do not associate to the 38 kDa protein which complexes with pp60 super(c src) and pp60 super(c-srcN)

• Published in: FEBS letters Vol. 332; no. 1-2; pp. 27 - 30
• Main Authors: Meyerson, G, Pahlman, S
• Format: Journal Article
• Language: English
• Published: 01-01-1993
• ISSN: 0014-5793

The tyrosine-specific kinases pp60 super(c-src) and pp60 super(c-srcN) (pp60 super(src)) are slightly enriched and activated in growth cones isolated from neuronally differentiating SH-SY5Y neuroblastoma cells. In the growth cones the two src isoforms are associated with a 38 kDa protein. In this report, we have compared the subcellular distribution of pp59 super(fyn) and pp62 super(fyn) and pp62 super(c-yes) with that of pp60 super(src) in differentiating SH-SY5Y cells. Like pp60 super(src), the other two tyrosine kinases were slightly enriched and activated in the growth cones as compared to the levels in the cell bodies. The kinase activites were 3- to 4-times higher in growth cones than in cell bodies. However, only pp60 super(src) formed a complex with the 38 kDa protein while immunoprecipitation of pp59 super(fyn) brought down an additional protein of 90 kDa. This may suggest that these related tyrosine kinases have different substrates and in part mediate different cellular responses in the growth cones of differentiating SH-SY5Y cells.