Tuerkiye'de bulunan bazi keci irklarinda mikrosatellit DNA markorlerinin kimliklendirme calismalarinda kullanilabilirliginin arastirilmasi/Usefullness of Microsatellite DNA Markers for Parentage Testing for Some Goat Populations in Turkey

• Published in: Journal of cell and molecular biology Vol. 12; no. 1-2; p. 39
• Main Authors: Bulut, Zafer, Kurar, Ercan, Oezsensoy, Yusuf, Altunok, Vahdettin, Nizamlioglu, Mehmet
• Format: Journal Article
• Language: English
• Published: 01-12-2014
• ISSN: 1303-3646; 1306-0961

Characterization of populations at the molecular level provides an estimation of genetic diversity and distances between and within the populations. For this purpose, microsatellites are widely preferred marker systems in population analyses. Genotypic data at DNA level were also used to test genetic relationships between individuals in a population and for practical verification of paternity. The objective of this study was to evaluate the usefulness of microsatellite markers for parentage testing for some goat breeds in Turkey. The DNA samples were isolated by using a standard organic based method. Eleven microsatellite loci were selected from a list suggested by a joint ISAG/FAO working group. Microsatellite markers were used to amplify genomic DNA by polymerase chain reaction (PCR). The amplified PCR products were separated and fragment analyses were accomplished by capillary electrophoresis. Allele numbers, observed heterozygosity, expected heterozygosity, deviation from Hardy-Weinberg Equilibrium and probability of exclusion at each microsatellite locus were calculated. Allele numbers were determined ranging from 3 to 25 at each locus.