BIOLOGICAL INTERACTIONS OF MUSCLE PRECURSOR C2C12 CELLS WITH BIOMIMETIC NANO-HYDROXYAPATITE/POLY(LACTIDE-CO-GLYCOLIDE) SCAFFOLDS

• Published in: Ceramics international Vol. 40; no. 9; pp. 14305 - 14311
• Main Authors: Amna, T, Hassan, M S, Khil, M-S, Hwang, I
• Format: Journal Article
• Language: English
• Published: 01-01-2014
• Subjects: Assaying; Biological; Diseases; Electrospinning; Muscles; Nanostructure; Precursors; Scaffolds; Scanning electron microscopy
• ISSN: 0272-8842

The biological interactions of muscle precursor C2C12 cells on biomimetic nano-hydroxyapatite/poly(lactide-co-glycolide) scaffold surfaces were studied. The nanofibrous scaffolds containing hydroxyapatite (HAP) particles were synthesised by sol-gel elecrospinning for the seeding and growth of muscle cells. The as-spun hybrid nanofibres were characterised by SEM, TEM/EDX and XRD and thermal stability of the composite scaffolds was determined by TGA. The cytobiocompatibility of mouse C2C12 myoblasts on the matrix was examined using a cell counting assay. Results revealed that incorporation of HAP in the polymer affected both the morphology and size of polylactide-co-glycolide nanofibres. The CCK-8 assay results and SEM observation showed that the composite nanofibrous scaffolds were non-cytotoxic to C2C12 cell culture and enabled cell attachment and proliferation. Biomimetic scaffolds, with homogeneously distributed HAP nanoparticles, were fabricated by electrospinning. Results suggest that the HAP/poly(lactide-co-glycolide) scaffolds are promising materials for the growth of C2C12 cells and thus helps in the development of novel therapeutic systems for skeletal muscle diseases in the future.