Saponin adjuvant induction of ovalbumin-specific CD8 super(+) cytotoxic T lymphocyte responses

Saponin adjuvant induction of ovalbumin-specific CD8 super(+) cytotoxic T lymphocyte responses

The ability of a saponin adjuvant, QS-21, to induce OVA-specific, class I MHC Ag-restricted CTL was investigated using different forms of soluble OVA and OVA adsorbed onto alum as immunogens. C57BL/6 mice were immunized with soluble native or denatured OVA in formulations that contained increasing q...

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Bibliographic Details
Published in:The Journal of immunology (1950) Vol. 148; no. 8; pp. 2357 - 2362
Main Authors: Newman, MJ, Wu, Jia-Yan, Gardner, B H, Munroe, K J, Leombruno, D, Recchia, J, Kensil, C R, Coughlin, R T
Format: Journal Article
Language:English
Published: 01-01-1992
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Summary:The ability of a saponin adjuvant, QS-21, to induce OVA-specific, class I MHC Ag-restricted CTL was investigated using different forms of soluble OVA and OVA adsorbed onto alum as immunogens. C57BL/6 mice were immunized with soluble native or denatured OVA in formulations that contained increasing quantities of QS-21, and CTL responses were measured using EL4 and E.G7-OVA cells as targets and splenic mononuclear cells as effector. The CTL activity of splenic mononuclear cell preparations was totally destroyed by treatment with mAb specific to the CD8 Ag plus complement. The ability of the QS-21 adjuvant to induce class I MHC Ag-restricted CTL after immunization with soluble proteins is a characteristic unique to saponin adjuvants.
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ISSN:0022-1767