Readthrough transcription occurs at the rho dependent signal F1 T sub(IV) in suppressor cells

• Published in: Nucleic acids research Vol. 18; no. 4; pp. 865 - 870
• Main Authors: La Farina, M, Izzo, V, Costa, MA, Barbier, R, Duro, G, Vitale, M, Mutolo, V
• Format: Journal Article
• Language: English
• Published: 01-01-1990
• Subjects: phage f1
• ISSN: 0305-1048

Suppressor cells infected with bacteriophage f1 yield phage encoded gene IV transcripts longer than those present in the sup degree host and identical to those found in a rho super(-) host. However, such longer transcripts do not appear in the suppressor-infected cell when, by changing the translation frame of gene IV, the ribosome is not allowed to proceed to the end of the gene IV message and thus to reach the rho dependent transcription terminator f1 T sub(IV). This suggests that ribosome movement beyond the natural gene IV stop codon disturbs the activity of that termination signal.