In vitro [alpha]-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit

• Published in: Asian Pacific journal of tropical biomedicine Vol. 4; no. 6; pp. 473 - 479
• Main Authors: Uddin, Nizam, Hasan, Md Rakib, Hossain, Md Monir, Sarker, Arjyabrata, Hasan, A HM Nazmul, Islam, A FM Mahmudul, Chowdhury, Mohd Motaher H, Rana, Md Sohel
• Format: Journal Article
• Language: English
• Published: 01-06-2014
• Subjects: Citrus
• ISSN: 2221-1691
• DOI: 10.12980/APJTB.4.2014C1173

Objective: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr. fruit in [alpha]-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo). Methods: Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, [alpha]-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats. Results: Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate [alpha]-amylase inhibitory activity [IC sub(50) value=(3.638+ or -0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1 000 mg/kg doses fruit extract significantly (P<0.05 and P<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (P<0.05) at 2 h but 1000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (P<0.05 and P<0.01 respectively) whereas glibenclamide (5 mg/ kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test. Conclusion: These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.