Characterization of FMRFamide-Related Peptides from the osmoregulatory tissues of the pulmonate snail, Helisoma trivolvis
Three FMRFamide-Related Peptides (FaRPs) were purified and identified from kidney extracts from the snail, Helisoma trivolvis by High Performance Liquid Chromatography (HPLC), using four different radioimmunoassays (RIAs) against FaRPs to detect the immunoreactive fractions. Automated sequencing (Ed...
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| Format: | Dissertation |
| Language: | English |
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ProQuest Dissertations & Theses
01-01-1993
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| Online Access: | Get full text |
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| Summary: | Three FMRFamide-Related Peptides (FaRPs) were purified and identified from kidney extracts from the snail, Helisoma trivolvis by High Performance Liquid Chromatography (HPLC), using four different radioimmunoassays (RIAs) against FaRPs to detect the immunoreactive fractions. Automated sequencing (Edman degradation) and mass spectrometry show that these three purified peptides were: Phe-Met-Arg-Phe-NH$\sb2$ (FMRFamide), Phe-Leu-Arg-Phe-NH$\sb2$ (FLRFamide) and Gly-Asp-Pro-Phe-Met-Arg-Phe-NH$\sb2$ (GDPFLRFamide). HPLC and RIA were used to determine the distribution of these three FaRPs in the hemolymph and two other major osmoregulatory tissues, the skin and mantle. FMRFamide-like material has been previously localized in the central nervous system (CNS) and in the kidney of Helisoma duryi by immunohistochemical techniques. The present study suggests that FaRPs are involved in osmoregulation of Helisoma trivolvis. (Abstract shortened by UMI.) |
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| ISBN: | 0315841796 9780315841796 |