Proline Rich Polypeptide (PRP-1) Increases the Superoxide-Producing and Ferrihemoglobin Reducing Activities of Cytochrome B^sub 558^ Isoforms from Human Lymphosarcoma Tissue Cells

• Published in: Neurochemical research Vol. 36; no. 5; p. 739
• Main Authors: Simonyan, G M, Galoian, K A, Simonyan, R M, Simonyan, M A, Galoyan, A A
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01-05-2011
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-010-0389-7

The two cytochromes (cyt) b^sub 558^ of acidic nature, one--95-100 kDa and another one, 60-70 kDa were isolated for the first time from the human's lymphosarcoma tissue cells using gel filtration and ion exchange chromatography. These hemoproteins possess NADPH dependent O2 ^sup -^-producing and ferrihemoglobin-reducing activities. The incubation of neuropeptide PRP-1 (5 μg) with cytochrome b^sub 558^, caused elevation of these activities. The gel filtration results indicated possible binding of PRP-1 to these cytochromes b^sub 558^. PRP-1 activated both NADPH dependent O2 ^sup -^-producing and ferriHb-reducing activities of the cyt b^sup 1^ ^sub 558^ and cyt b^sup 2^ ^sub 558^, obtained from human lymphosarcoma tissue cells. One can assume that PRP-1 associated with cyt b^sub 558^ on the surface of the tumor cells by increasing both NADPH dependent O2 ^sup -^-producing and ferriHb-reducing activities of cyt b^sub 558^, increases the oxidation- reduction status. Changing the oxidation-reduction status and oxygen homeostasis of the tumor cells by PRP-1 can serve as one of the possible explanation of antitumorigenic effect of this cytokine.[PUBLICATION ABSTRACT]