Role of Neuropeptide QRFP in the Hypothalamus

• Main Author: Olga, Zagorácz
• Format: Dissertation
• Language: English
• Published: ProQuest Dissertations & Theses 01-01-2021
• Subjects: Adrenergic receptors; Anxiety; Behavioral psychology; Brain research; Clinical psychology; Cognition & reasoning; Cognitive psychology; Communication; Endocrinology; European history; Food; Glucose; Histology; Hormones; Hypothalamus; Memory; Mental disorders; Mental health; Metabolism; Neurons; Neuropeptides; Neurosciences; Physiology; Pituitary gland; Post traumatic stress disorder; Regulation; Renaissance period; Serotonin; Sexual behavior; Sexuality
• ISBN: 9798384321415

Summary Summarizing our data, the following results have been received:• The MHA administration of QRFP in doses 100 and 200 ng led to a significant and rapid increase in food consumption. The orexigenic effect was attenuated, and for a short period even taken over into opposite direction, by an equimolar dose of receptor antagonist BIBP3226 (Ant).• The LHA administration of QRFP in doses 100 and 200 ng led to a significant and rapid decrease in food consumption. The anorexigenic effect was abolished by an equimolar dose of the Ant.Thus, QRFP microinjections into the MHA increase, while microinjections into the LHA decrease food intake. The Ant prevents these effects.• The MHA administration of QRFP in doses 200 and 400 ng, as well as Ant in corresponding dose, did not induce place preference in the CPP paradigm.• The LHA administration of QRFP in doses 200 and 400 ng, did not induce place preference in the CPP paradigm. Ant administration into the LHA led to significantly longer time spent in the TQ, which is considered as anxiogenic sign.This way, neither QRFP, nor Ant induced place preference when applied into the MHA or the LHA.• The MHA administration of QRFP in doses 200 and 400 ng improved consolidation of memory in the MWM test. The effect was abolished by an equimolar dose of the Ant.• The LHA administration of QRFP in doses 200 and 400 ng promoted memory consolidation in the MWM test. The effect was abolished by an equimolar dose of the Ant.These data suggest that QRFP administration into both the MHA and the LHA improve short-term memory. The Ant prevents these effects.• The MHA administration of QRFP in doses 200 and 400 ng did not affect anxiety in the EPM test. Ant in corresponding dose caused an anxiogenic effect.• The LHA administration of QRFP in doses 200 and 400 ng did not affect anxiety in the EPM test. Ant in corresponding dose caused an anxiogenic effect.QRFP microinjections into the MHA and the LHA did not change anxiety level. These data prove that feeding- and learning-modifying effects of the QRFP were not affected by the changes in anxiety status.• The MHA administration of QRFP in doses 200 and 400 ng, as well as Ant, did not affect general locomotor activity according to the OFT.• The LHA administration of QRFP in doses 200 and 400 ng did not affect general locomotion according to the OFT. Ant did not change locomotor activity, except for the acceleration of the grooming activity, which we tend to account as a sign of anxiety.QRFP administration into the MHA and the LHA did not affect general locomotion. These finding prove that feeding- and learning-modifying effects of QRFP were not induced by hyper- or hypo locomotion.We have shown with this research that hypothalamic neuropeptide QRFP has a great potential as an application point for treatment development in multiple spheres. On one side, dual character of feeding regulation by QRFP depending on application sight, i.e., satiety or hunger center, opens doors for treatment of various feeding disorders from anorexia to binge eating and obesity.