Chemical Profiling of Paddy Husk and its Inhibitory Activity against Human Salivary Gland Cancer

Chemical Profiling of Paddy Husk and its Inhibitory Activity against Human Salivary Gland Cancer

Global agriculture produces tens of millions of tons of paddy husk every year. Paddy husk is an inedible agriculture waste obtained during the process of rice milling. Previous studies reported that it contains inhibitory potential due to the presence of phytochemicals that possess anti-cancer prope...

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Bibliographic Details
Published in:Asia-Pacific journal of molecular biology and biotechnology Vol. 31; p. 29
Main Authors: Al-azazi, Entesar Ahmed, Yusop, Norhayati, Murtey, Mogana Das
Format: Journal Article
Language:English
Published: Kuala Lumpur Malaysian Society for Molecular Biology and Biotechnology 01-06-2023
Subjects:
Agricultural wastes
Agriculture
Anticancer properties
Cancer
Cell number
Cell viability
Cytotoxicity
Exocrine glands
Methanol
Phytochemicals
Salivary gland
Salivary glands
Tocopherol
Toxicity testing
Tumors
Vitamin E
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Summary:Global agriculture produces tens of millions of tons of paddy husk every year. Paddy husk is an inedible agriculture waste obtained during the process of rice milling. Previous studies reported that it contains inhibitory potential due to the presence of phytochemicals that possess anti-cancer properties. The main aim of this study is to identify the presence of anti-cancer-related phytochemicals in paddy husk aqueous methanol extract and elucidate its inhibitory effects against human submaxillary salivary gland epidermoid carcinoma cells (HTB-41).Chemical profiling of paddy husk aqueous methanol was done by using GC/MS analysis while the inhibitory activity (IC50) against HTB-41 cells was calculated by using the Trypan Blue Exclusion Assay (TBEA).Paddy husk aqueous methanol profiling analysis shows the presence of vitamin E such as tocopherol which possessed anti-cancer properties. Paddy husk aqueous methanol elucidated inhibitory activity on HTB-41 cells where an IC50 dose of 200 μg/ml managed to reduce cell viability to 50.5%. This extract act in a tumor-selective manner since the cytotoxicity test showed no significant changes (88.90%) in cell number and viability after treatment. The presence of vitamin E in paddy husk aqueous methanol successfully showed inhibitory effects on the human salivary cancer cell line while it acted in a tumor-selective manner by not inducing any significant changes in human gingival fibroblast cell (HGF-1).
ISSN:0128-7451