Antimicrobial activity and phytochemical profile of Acacia drepanolobium Harms Ex Sjostedt (Fabaceae) and Solanum arundo Mattei (Solanaceae)

Antimicrobial activity and phytochemical profile of Acacia drepanolobium Harms Ex Sjostedt (Fabaceae) and Solanum arundo Mattei (Solanaceae)

Materials And Methods Plant collection and authentication Various plant parts, including leaves, stem barks and roots of Acacia drepanolobium and leaves, stems, and roots of Solanum arundo, were collected in January 2019, from Makuyuni ward in Monduli District, Arusha region, with the help of a bota...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences and research Vol. 12; no. 5; pp. 734 - 739
Main Authors: Mollel, Emburis, Runyoro, Deborah Kiumlo Balira, Ngassapa, Olipa David
Format: Journal Article
Language:English
Published: Cuddalore Journal of Pharmaceutical Sciences and Research 01-05-2020
Subjects:
Alzheimer's disease
Antimicrobial agents
Bacteria
Diarrhea
Drug resistance
Flavonoids
Fungal infections
Herbal medicine
Low income groups
Microorganisms
Mortality
Phytochemicals
Pneumonia
R&D
Research & development
Tanzania
India
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Summary:Materials And Methods Plant collection and authentication Various plant parts, including leaves, stem barks and roots of Acacia drepanolobium and leaves, stems, and roots of Solanum arundo, were collected in January 2019, from Makuyuni ward in Monduli District, Arusha region, with the help of a botanist from Tanzania Pesticide Research Institute (TPRI). Dried ground plant material (100g) of each plant part, was extracted exhaustively by maceration with methanol at room temperature, and the extracts were concentrated at 50 C in vacuo using Buchi rotary evaporator. Screening for Antimicrobial activity Standard medicines, solvents, reagents and media Ciprofloxacin, fluconazole, P-iodonitrotetrazolium (INT) chloride dye and Sabouraud Dextrose Broth all from Sigma Aldrich (Germany), methanol (Blulux Laboratories ltd India ), ethylacetate, petroleum ether and dichloromethane all from Lobal Chemie ltd India, DMSO (Carlo Erba Spain), Sabouraud Dextrose Agar (Liofilchem Italy) and Mueller Hinton Broth (Oxoid Ltd, UK) were used in this study. Test organisms Test organisms included standard strains of Escherichia coli (ATCC25922), Klebsiella pneumoniae (ATCC708903), Staphylococcus aureus (ATCC25923), Pseudomonas aeruginosa (ATCC27853), Salmonella typhi (ATCC19430), Proteus vulgaris (ATCC33420) and Candida albicans (ATCC10231). inocula Each organism was cultured separately; bacteria were subcultured repeatedly on Mueller Hinton (Oxoid Ltd) agar slants at 37 C for 24 hrs.
ISSN:0975-1459