Comparing number and intensity of doublecortin-immunolabelled cells in the hippocampus and cerebellum in the postnatal mice brains, in different age groups

• Published in: Journal of pharmaceutical sciences and research Vol. 11; no. 5; pp. 1948 - 1953
• Main Author: Saadoon, Zainab Zahid
• Format: Journal Article
• Language: English
• Published: Cuddalore Journal of Pharmaceutical Sciences and Research 01-05-2019
• Subjects: Age groups; Brain; Cell division; Neurogenesis; Neurons; Neurosciences; Proteins; Rodents; Stem cells
• ISSN: 0975-1459

In this study we investigated the expression of doublecortin immunolabelled cells in both dentate gyrus of hippocampus of adult mice brain and in cerebellum of the first four days postnatal mice brains and correlated those expressions with successive age groups of both, by immunolabelling of doublecortin on series of paraffin sections. In adult hippocampal neurogenesis DCX marks the period between the committed progenitor cell stages (type-2b/3) and the early postmitotic maturation stage and is absent from the radial-glia-like stem cells (type-1), the non-committed progenitor cells (type-2a) and the mature neurons (Couillard-Despres et al. 2005). In this study we investigated the number of positive expression of doublecortin immunolabelled cells and the intensity of this positivity in both dentate gyrus of hippocampus of adult mice brains and cerebellum of 0 day, 2 days and 4 days postnatal mice brains and correlated those expressions with age by immunolabelling of doublecortin on series of paraffin sections. DCX signifies transient neuronal lineage commitment together with migration and neural structural plasticity in the adult hippocampal niche, and postnatal cerebellum. this is necessary for rapid adaptation to environmental changes, and indicate continuous plasticity in early life and may be throughout life in hippocampus (extended age group study required).