SPCA2 couples Ca^sup 2+^ influx via Orai1 to Ca^sup 2+^ uptake into the Golgi/secretory pathway

• Published in: Tissue & cell Vol. 49; no. 2 Part A; p. 141
• Main Authors: Smaardijk, Susanne, Chen, Jialin, Wuytack, Frank, Vangheluwe, Peter
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Science Ltd 01-04-2017
• Subjects: Adenosine triphosphatase; Breast cancer; Breastfeeding & lactation; Calcium channels; Calcium influx; Calcium ions; Calcium transport; Cancer; Cells; Golgi apparatus; Lactation; Mammary gland; Mammary glands; Orai1 protein; Transport
• ISSN: 0040-8166; 1532-3072

Dysregulation of the Golgi/Secretory Pathway Ca2+ transport ATPase SPCA2 is implicated in breast cancer. During lactation and in luminal breast cancer types, SPCA2 interacts with the plasma membrane Ca2+ channel Orai1, promoting constitutive Ca2+ influx, which is termed store independent Ca2+ entry (SICE). The mechanism of SPCA2/Orai1 interaction depends on the N- and C-termini of SPCA2. These extensions may play a dual role in activating not only Orai1, but also Ca2+ transport into the Golgi/secretory pathway, which we tested by investigating the impact of various SPCA2 N- and/or C-terminal truncations on SICE and Ca2+ transport activity of SPCA2. C-terminal truncations impair SICE and SPCA2 activity, but also affect targeting, whereas N-terminal truncations affect targeting and inactivate SPCA2, but remarkably, SICE activation remains unaffected. Importantly, overexpression of SPCA2 increases the Ca2+ content of non-ER stores, which depends on Orai1 and SPCA2 activity. Thus, Orai1-mediated Ca2+-influx and SPCA2-mediated Ca2+ uptake activity into the Golgi/secretory pathway might be coupled possibly in a microdomain. This channel/pump complex may efficiently transfer Ca2+ into the secretory pathway, which might play a role in SPCA2-expressing secretory cells, such as mammary gland during lactation.