Influence of Cdx2 and TaqI Gene Variants on Vitamin D3Modulated Intracellular Chemokine Positive T-Cell Subsets in Pulmonary Tuberculosis

• Published in: Clinical therapeutics Vol. 39; no. 5; p. 946
• Main Authors: Murugesan Harishankar, Selvaraj, Paramasivam
• Format: Journal Article
• Language: English
• Published: Bridgewater Elsevier Limited 01-05-2017
• Subjects: Blood; Calcitriol; CDX2 protein; Cell activation; Cell culture; Chemokines; Chlorophyll; Cytokines; Drugs; Filtrate; Fragmentation; Gene polymorphism; Genes; Genotypes; Genotyping; Granulocytes; Immunoglobulins; Infections; Inflammatory response; Interferon; Intracellular; IP-10 protein; Lymphocytes; Lymphocytes T; Macrophages; Monocyte chemoattractant protein; Monocyte chemoattractant protein 1; Patients; Polymerase chain reaction; Polymorphism; Proteins; RANTES; Restriction fragment length polymorphism; Tuberculosis; Vitamin D; Vitamin D3; Vitamin deficiency; γ-Interferon; India
• ISSN: 0149-2918; 1879-114X
• DOI: 10.1016/j.clinthera.2017.04.003

Purpose We studied the effect of 1,25(OH)2D3(vitamin D3) on intracellular chemokine-positive T-cell subsets in whole blood cultures of healthy controls and patients with pulmonary tuberculosis. Methods Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism method. The regulatory role of theCdx2and 3ʹUTRTaqIgene variants on chemokine-positive T-cell subsets was studied from culture filtrate antigen stimulated with or without vitamin D3treated whole blood cultures of 60 healthy controls and 50 patients with pulmonary tuberculosis. Findings Vitamin D3significantly suppressed monocyte chemoattractant protein 1, macrophage inhibitory protein (MIP)-1α, MIP-1β, regulated on activation, normal T-cell expressed and secreted (RANTES), and interferon-γ inducible protein 10 (IP-10)-positive T-cell subsets compared with culture filtrate antigen stimulated cells without vitamin D3treatment. In theCdx2AA genotype, vitamin D3decreased MIP-1α, MIP-1β, and RANTES-positive T cells compared with the GG genotype. Whereas in theTaqItt genotype, decreased MIP-1β and RANTES and increased IP-10-positive T cells were observed compared with the TT genotype in vitamin D3treated cells (p< 0.05). Implications This study suggests that vitamin D3may regulate the chemokine-positive T cells through theCdx2AA andTaqItt genotypes. This could be helpful to regulate chemokine-mediated inflammatory response during active disease condition. Hence, vitamin D3supplementation along with tuberculosis drugs may be useful for faster recovery from the disease.