Effect of Ionophores on Activity of Na^sup +^,Cl^sup -^(HCO[Stack ^sub 3^ ^sup -^ ])-ATPase

• Published in: Bulletin of experimental biology and medicine Vol. 161; no. 6; p. 763
• Main Authors: Yurkiv, V A, Melikhov, V I, Shubin, V S
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01-10-2016
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-016-3504-1

We studied the effects of ionophores on activity of Na^sup +^,Cl^sup -^(HCO[Stack ^sub 3^ ^sup -^ ])-ATPase. The most significant effect on the activity of this enzyme was produced by protonophore 2,4-dinitrophenol. The effect of this drug largely depended on the cation and anion composition and pH of the incubation medium and its pH. Activity of Na^sup +^,Cl^sup -^(HCO[Stack ^sub 3^ ^sup -^ ])-ATPase increased at neutral and weakly alkaline pH and decreased at pH below 6.5-6.7. In control animals (without histamine injection) with very weak or absent Na^sup +^,Cl^sup -^(HCO[Stack ^sub 3^ ^sup -^ ])-ATPase activity, the observed effect of the protonophore on ATPase activity was also virtually absent. The stimulatory effect of other ionophores (monensine, valinomycin, and A^sub 23187^) was significantly weaker and depended on pH of the incubation medium, its cationic and anionic composition, and concentration of these ionophores.