Specific immunotherapy generates CD8^sup +^ CD196^sup +^ T cells to suppress lung cancer growth in mice

• Published in: Immunologic research Vol. 64; no. 4; p. 1033
• Main Authors: Zhang, Jian, Liu, Jing, Chen, Huiguo, Wu, Weibin, Li, Xiaojun, Wu, Yonghui, Wang, Zhigang, Zhang, Kai, Li, Yun, Weng, Yimin, Liao, Hongying, Gu, Lijia
• Format: Journal Article
• Language: English
• Published: Totowa Springer Nature B.V 01-08-2016
• Subjects: Cellular biology; Immunotherapy; Lung cancer; Lymphocytes; Rodents
• ISSN: 0257-277X; 1559-0755
• DOI: 10.1007/s12026-016-8793-y

That specific immunotherapy can inhibit cancer growth has been recognized; its efficiency is to be improved. This study aimed to inhibit lung cancer (LC) growth in a mouse model by using an LC-specific vaccination. In this study, a LC mouse model was created by adoptive transplantation with LC cells. The tumor-bearing mice were vaccinated with LC cell extracts plus adjuvant TNBS or adoptive transplantation with specific CD8+ CD196+ T cells. The results showed that the vaccination with LC extracts (LCE)/TNBS markedly inhibited the LC growth and induced CD8+ CD196+ T cells in LC tissue and the spleen. These CD8+ CD196+ T cells proliferated and produce high levels of perforin upon exposure to LCE and specifically induced LC cell apoptosis. Exposure to TNBS induced RAW264.7 cells to produce macrophage inflammatory protein-3[alpha]; the latter activated signal transducer and activator of transcription 3 and further induced perforin expression in the CD8+ CD196+ T cells. Adoptive transfer with specific CD8+ CD196+ T cells suppressed LC growth in mice. In conclusion, immunization with LC extracts and TNBS can induce LC-specific CD8+ CD196+ T cells in LC-bearing mice and inhibit LC growth.