Oxygen stress: impact on innate immune system, antioxidant defence system and expression of HIF-1[alpha] and ATPase 6 genes in Catla catla

• Published in: Fish physiology and biochemistry Vol. 42; no. 2; p. 673
• Main Authors: Singh, Samar Pal, Sharma, Jaigopal, Ahmad, Tauqueer, Chakrabarti, Rina
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01-04-2016
• Subjects: Antioxidants; Climate change; Consumption; Dissolved oxygen; Gene expression; Gills; Hyperoxia; Hypoxia; Immune system; Metabolism; Muscles; Nitric oxide; Organisms; Oxidative stress; Oxygen; India
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-015-0168-0

Catla catla catla (2.28 ± 0.1 g) were exposed to six different levels of dissolved oxygen: 1 (DO-1), 3 (DO-3), 5 (DO-5), 7 (DO-7), 9 (DO-9) and 11 (DO-11) mg/L. DO-5 served as control. In DO-1 and DO-3, the number of red blood cells (RBC), lysozyme, respiratory burst activity and nitric oxide synthase were significantly (p < 0.05) lower compared to the control one. In DO-7 and DO-9, RBC and lysozyme were significantly (p < 0.05) higher compared to the control one. Thiobarbituric acid reactive substances was significantly (p < 0.05) higher in catla exposed at low (1 and 3 mg/L) and high (9 and 11 mg/L) dissolved oxygen compared to others. In muscles and hepatopancreas, reduced glutathione was significantly (p < 0.05) higher in DO-5 and DO-7 and in gills of DO-5 compared to others after 1 h. In muscles, glutathione S-transferase (GST) was significantly (p < 0.05) lower in DO-5 and DO-7 compared to others. In hepatopancreas, GST and glutathione peroxidise (GPx) were significantly (p < 0.05) higher in DO-1 and DO-3 compared to others. In gills, GPx was higher in DO-9 and DO-11 after 48 h. In brain, hypoxia-inducible factor (HIF)-1[alpha] mRNA level was induced in DO-1 and DO-3 compared to others after 1 h of exposure. In gills and hepatopancreas, HIF-1[alpha] mRNA level was significantly (p < 0.05) higher in DO-1 compared to others after 1 h. The ATPase 6 mRNA level was significantly (p < 0.05) higher in brain and hepatopancreas of DO-1 after 1 h and in gills and hepatopancreas of DO-3 and DO-9, respectively, after 48 h compared to others.