Interleukin-12 induces a Th1-like response toBurkholderia malleiand limited protection in BALB/c mice

• Published in: Vaccine Vol. 24; no. 9; p. 1413
• Main Authors: Amemiya, Kei, Meyers, Jennifer L, Trevino, Sylvia R, Chanh, Tran C, Norris, Sarah L, Waag, David M
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Limited 27-02-2006
• Subjects: Cell culture; Cytokines; Glycerol; Immune response; Immune system; Infections; Laboratory animals; Medical research; Studies; Vaccines
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2005.09.021

We evaluated the effect of interleukin (IL)-12 on the immune response toBurkholderia malleiin BALB/c mice. Mice were vaccinated with non-viableB. malleicells with or without IL-12. There was a seven- to nine-fold increase in IgG2a levels, and a significant increase in the proliferative response and interferon (IFN)-γ production by splenocytes from mice that receivedB. malleiand IL-12. We saw an increase in survivors in the groups of mice that receivedB. malleiand IL-12 when challenged, compared to mice that received onlyB. malleior IL-12. The results suggest that IL-12 can enhance the Th1-like immune response toB. malleiand mediate limited protection from a lethal challenge.