Efficacy of commercial and field-strainMycobacterium paratuberculosisvaccinations with recombinant IL-12 in a bovine experimental infection model

• Published in: Vaccine Vol. 21; no. 23; p. 3101
• Main Authors: Uzonna, Jude E, Chilton, Paula, Whitlock, Robert H, Habecker, Perry L, Scott, Phillip, Sweeney, Raymond W
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Limited 04-07-2003
• Subjects: Cattle; Milk; Pasteurization; Vaccines; United States > US
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(03)00261-5

The efficacy of commercial (Strain 18) and field-isolate paratuberculosis vaccine preparations was investigated. The effect of prior exposure toMycobacterium paratuberculosisand the adjuvant effect of rIL-12 on vaccine efficacy were also tested. Both Strain 18 and field-isolate vaccines induced strong local, systemic and enteric IFN-γ responses. A significant reduction in mycobacterial colonization was observed when calves were vaccinated with the field-isolate prior to challenge, but not following vaccination with Strain 18 vaccine. Vaccination with rIL-12 prevented infection in some calves but its overall effect on IFN-γ response and total mycobacterial load was not statistically significant. Efficacy ofparatuberculosisvaccines may be enhanced if calves are vaccinated prior toM. paratuberculosisexposure with field-isolate vaccine instead of Strain 18 vaccine currently in use.