Asn^sub 3^, a Reliable, Robust, and Universal Lock Mass for Improved Accuracy in LC-MS and LC-MS/MS

Asn^sub 3^, a Reliable, Robust, and Universal Lock Mass for Improved Accuracy in LC-MS and LC-MS/MS

The use of internal calibrants (the so-called lock mass approach) provides much greater accuracy in mass spectrometry based proteomics. However, the polydimethylcyclosiloxane (PCM) peaks commonly used for this purpose are quite unreliable, leading to missing calibrant peaks in spectra and correspond...

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Bibliographic Details
Published in:Analytical chemistry (Washington) Vol. 85; no. 22; p. 11054
Main Authors: Staes, An, Vandenbussche, Jonathan, Demol, Hans, Goethals, Marc, Yilmaz, Sule, Hulstaert, Niels, Degroeve, Sven, Kelchtermans, Pieter, Martens, Lennart, Gevaert, Kris
Format: Journal Article
Language:English
Published: Washington American Chemical Society 19-11-2013
Subjects:
Biochemistry
Mass spectrometry
Organic chemicals
Peptides
Proteomics
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Summary:The use of internal calibrants (the so-called lock mass approach) provides much greater accuracy in mass spectrometry based proteomics. However, the polydimethylcyclosiloxane (PCM) peaks commonly used for this purpose are quite unreliable, leading to missing calibrant peaks in spectra and correspondingly lower mass measurement accuracy. Therefore, we here introduce a universally applicable and robust internal calibrant, the tripeptide Asn3. We show that Asn3 is a substantial improvement over PCM both in terms of consistent detection and resulting mass measurement accuracy. Asn3 is also very easy to adopt in the lab, as it requires only minor adjustments to the analytical setup. [PUBLICATION ABSTRACT]
ISSN:0003-2700
1520-6882