Cover Picture: Chemical Protein Synthesis by Chemoselective [alpha]-Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline (Angew. Chem. Int. Ed. 21/2012)
The chemical synthesis of proteins from unprotected peptide segments uses KAHA ligation with 5-oxaproline, which is incorporated readily into peptides by solid-phase peptide synthesis. Ligation of such protein segments in aqueous buffers with an α-ketoacid peptide gives an α-homoserine residue at th...
Saved in:
| Published in: | Angewandte Chemie International Edition Vol. 51; no. 21; p. 5015 |
|---|---|
| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Weinheim
Wiley Subscription Services, Inc
21-05-2012
|
| Edition: | International ed. in English |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The chemical synthesis of proteins from unprotected peptide segments uses KAHA ligation with 5-oxaproline, which is incorporated readily into peptides by solid-phase peptide synthesis. Ligation of such protein segments in aqueous buffers with an α-ketoacid peptide gives an α-homoserine residue at the ligation site. The new ligation is used for the synthesis of two proteins, prokaryotic-ubiquitin like protein (Pup) and probable cold shock protein A (cspA; see scheme). |
|---|---|
| ISSN: | 1433-7851 1521-3773 |
| DOI: | 10.1002/anie.201202064 |