Extracellular [beta]-D-glucosidase of the Penicillium canescens marine fungus

Extracellular [beta]-D-glucosidase of the Penicillium canescens marine fungus

Extracellular β-D-glucosidase was isolated in a homogeneous state from the Penicillium canescens marine fungus. According to SDS-electrophoresis, the molecular weight of the enzyme was 64 kDa and the maximal activity was observed at pH 5.2 and 70°C. Glucosidase catalyzed the hydrolysis of β-glycosid...

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Bibliographic Details
Published in:Applied biochemistry and microbiology Vol. 48; no. 4; p. 401
Main Authors: Dubrovskaya, Yu V, Sova, V V, Slinkina, N N, Anastyuk, S D, Pivkin, M V, Zvyagintseva, T N
Format: Journal Article
Language:English
Published: Dordrecht Springer Nature B.V 01-07-2012
Subjects:
Biochemistry
Enzymes
Fungi
Microbiology
Online Access:Get full text
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Summary:Extracellular β-D-glucosidase was isolated in a homogeneous state from the Penicillium canescens marine fungus. According to SDS-electrophoresis, the molecular weight of the enzyme was 64 kDa and the maximal activity was observed at pH 5.2 and 70°C. Glucosidase catalyzed the hydrolysis of β-glycosidic bonds both in glycosides and in glucose disaccharides and had transglycosylation activity. The enzyme can be used for the deglycosylation of natural glycosides and in enzymatic synthesis of new carbohydrate--containing compounds.[PUBLICATION ABSTRACT]
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683812040059