Application of Metal-Coded Affinity Tags (MeCAT): Absolute Proteir Quantification with Top-Down and Bottom-Up Workflows by Metal Coded Tagging
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| Published in: | Analytical chemistry (Washington) Vol. 84; no. 12; pp. 5268 - 5275 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
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Washington, DC
American Chemical Society
2012
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| Author | LINSCHEID, M. W AHRENDS, R NEUMANN, B SCHELER, C BERGMANN, U |
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| Author_xml | – sequence: 1 givenname: U surname: BERGMANN fullname: BERGMANN, U organization: Department of Chemistry, Humboldt-Universitaet zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany – sequence: 2 givenname: R surname: AHRENDS fullname: AHRENDS, R organization: Department of Chemistry, Humboldt-Universitaet zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany – sequence: 3 givenname: B surname: NEUMANN fullname: NEUMANN, B organization: Proteome Factory AG, Magnusstrasse 11, 12489 Berlin, Germany – sequence: 4 givenname: C surname: SCHELER fullname: SCHELER, C organization: Proteome Factory AG, Magnusstrasse 11, 12489 Berlin, Germany – sequence: 5 givenname: M. W surname: LINSCHEID fullname: LINSCHEID, M. W organization: Department of Chemistry, Humboldt-Universitaet zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany |
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| Keywords | Cysteine Lanthanide Peptides Internal standard Metal Inductive coupling plasma spectrometry Electrospray Reporter gene Fusion protein β-Galactosidase Quantitative analysis Flow injection Enzyme Coupled method Sample Chemical ionization Glycosylases Enzymatic method Residue Glycosidases Mass spectrometry MS/MS Proteomics Chelating agent Tagging Hydrolases Affinity Strategy Yield Two dimensional electrophoresis Application Mass spectrometry |
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| SubjectTerms | Analytical chemistry Biological and medical sciences Chemistry Diverse techniques Exact sciences and technology Fundamental and applied biological sciences. Psychology Molecular and cellular biology Spectrometric and optical methods |
| Title | Application of Metal-Coded Affinity Tags (MeCAT): Absolute Proteir Quantification with Top-Down and Bottom-Up Workflows by Metal Coded Tagging |
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